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Published May 15, 2005 | Published
Journal Article Open

Spectral-domain phase microscopy

Abstract

Broadband interferometry is an attractive technique for the detection of cellular motions because it provides depth-resolved phase information via coherence gating. We present a phase-sensitive technique called spectral-domain phase microscopy (SDPM). SDPM is a functional extension of spectral-domain optical coherence tomography that allows for the detection of nanometer-scale motions in living cells. The sensitivity of the technique is demonstrated, and its calibration is verified. A shot-noise limit to the displacement sensitivity of this technique is derived. Measurement of cellular dynamics was performed on spontaneously beating cardiomyocytes isolated from chick embryos.

Additional Information

© 2005 Optical Society of America Received October 8, 2004 This work was supported by National Institutes of Health grants R24-EB000243 and R21-RR019769 to J. A. Izatt and grants R01 HL715015 and P01 HL36059 to T. L. Creazzo.

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