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Published May 1, 1978 | public
Journal Article Open

RNA:DNA hybrids are more stable than DNA:DNA duplexes in concentrated perchlorate and trichloroacetate solutions

Abstract

Rates of formation of RNA:DNA hybrids have been measured as a function of temperature and compared to DNA:DNA duplex denaturation temperatures in 4 M sodium perchlorate, 4 M NaClO4-6 M urea, and 3 M rubidium trichioracetate solvents. The usual bell shaped curves of reaction rate versus temperature were observed. The optimal temperatures for the RNA:DNA association reaction are 5° to 12° greater than the Tm's for DNA:DNA denaturation in these solvents, just as in formamide. R-loops of φ80d3ilv DNA with E. coli rRNA can be formed at high efficiency in these solvents.

Additional Information

Copyright © 1978 Oxford University Press. Received 27 February 1978. We are grateful to Drs. R.L. Burke and W.R. Bauer for a generous gift of rubidium trichloracetate and to Drs. P. Chandler and J. Casey for advice. This research has been supported by contract N01 CP 43306 with the Virus Cancer Program of the National Cancer Institute. Y.H.C. has been supported in part by fellowship F32 CA 05099 from the National Cancer Institute.

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Additional details

Created:
August 22, 2023
Modified:
October 13, 2023