Published September 18, 1998
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Journal Article
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Identification of a Novel 81-kDa Component of the Xenopus Origin Recognition Complex
- Creators
- Carpenter, Phillip B.
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Dunphy, William G.
Abstract
The Xenopus origin recognition complex is essential for chromosomal DNA replication in cell-free extracts. We have immunopurified the Xenopus origin recognition complex with anti-Xorc2 antibodies and analyzed its composition and properties. Xorc2 (p63) is specifically associated with Xorc1 (p115) and up to four additional polypeptides (p81, p78, p45, and p40). The cDNA encoding p81 is highly homologous to various expressed sequence tags from humans and mice encoding a protein of previously unknown function. Immunodepletion of p81 from Xenopus egg extracts, which also results in the removal of Xorc2, completely abolishes chromosomal DNA replication. Thus, p81 appears to play a crucial role at S phase in higher eukaryotes.
Additional Information
Copyright © 1998 by the American Society for Biochemistry and Molecular Biology. (Received for publication, March 25, 1998, and in revised form, May 28, 1998) We thank Judith Campbell, Ray Deshaies, and members of the Dunphy laboratory for valuable comments during the course of this work. We are indebted to Wenying Shou for the generous gift of developmentally staged Xenopus embryos and to the California Institute of Technology Protein/Peptide Micro Analytical Laboratory for protein sequencing. We also thank Julian Blow and Alison Rowles for providing anti-Xorc1 antibodies. The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact. The nucleotide sequence(s) reported in this paper has been submitted to the GenBankTM/EMBL Data Bank with accession number(s) AF045607. {P.B.C. was] [s]upported by postdoctoral fellowships from the American Cancer Society and the E. S. Gosney Fund. {W.G.D. was] [s]upported by the Howard Hughes Medical Institute.Files
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- CaltechAUTHORS:CARjbc98
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