Redmond Red as a Redox Probe for the DNA-Mediated Detection of Abasic Sites
- Creators
- Buzzeo, Marisa C.
-
Barton, Jacqueline K.
Abstract
Redmond Red, a fluoropore containing a redox-active phenoxazine core, has been explored as a new electrochemical probe for the detection of abasic sites in double-stranded DNA. The electrochemical behavior of Redmond Red-modified DNA at gold surfaces exhibits stable, quasi-reversible voltammetry with a midpoint potential centered around −50 mV versus NHE. Importantly, with Redmond Red positioned opposite an abasic site within the DNA duplex, the electrochemical response is significantly enhanced compared to Redmond Red positioned across from a base. Redmond Red, reporting only if well-stacked in the duplex, represents a sensitive probe to detect abasic sites electrochemically in a DNA-mediated reaction.
Additional Information
Copyright © 2008 American Chemical Society. Received August 6, 2008; Revised Manuscript Received September 9, 2008. Publication Date (Web): October 3, 2008. We are grateful to the National Institutes of Health (GM 61077) for their financial support.Attached Files
Accepted Version - nihms-91115.pdf
Supplemental Material - BUZbc08supp.pdf
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Additional details
- PMCID
- PMC2747513
- Eprint ID
- 12558
- DOI
- 10.1021/bc800339y
- Resolver ID
- CaltechAUTHORS:BUZbc08
- NIH
- GM61077
- Created
-
2008-12-13Created from EPrint's datestamp field
- Updated
-
2021-11-08Created from EPrint's last_modified field