Published November 1, 2001
| public
Journal Article
Open
Objective-Type Dark-Field Illumination for Scattering from Microbeads
Chicago
An error occurred while generating the citation.
Abstract
We introduce a method for detecting and tracking small particles in a solution near a surface. The method is based on blocking the back-reflected illumination beam in an objective-type total internal reflection microscope, leaving unhindered the light scattered by the particles and resulting in dark-field illumination. Using this method, we tracked the motion of 60-nm polystyrene beads with a signal-to-noise ratio of 6 and detected 20-nm gold particles with a signal-to-noise ratio of 5. We illustrate the method's use by following the Brownian motion of small beads attached by short DNA tethers to a substrate.
Additional Information
© Copyright 2001 Optical Society of America Received 22 January 2001; revised manuscript received 6 July 2001. We acknowledge useful conversations with T. Kam, N. Davidson, M. Elbaum, and D. Bensimon and help from Y. Schopen.Files
BRAao01.pdf
Files
(814.4 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:95077d47c38df28fbf491cfdd425cbb8
|
814.4 kB | Preview Download |
Additional details
- Eprint ID
- 4573
- Resolver ID
- CaltechAUTHORS:BRAao01
- Created
-
2006-08-29Created from EPrint's datestamp field
- Updated
-
2020-02-20Created from EPrint's last_modified field