Published July 3, 1995
| Published
Journal Article
Open
Spiral Defects in Motility Assays: A Measure of Motor Protein Force
Abstract
In a commonly used motility assay, cytoskeletal filaments are observed as they glide over a surface coated with motor proteins. Defects in the motion frequently interrupt the flow of filaments. Examination of one such defect, in which a filament adopts a spiral form and rotates about a fixed point, provides a simple measure of the force exerted by the motor proteins. We demonstrate the universality of this approach by estimating the elementary forces of both myosin and kinesin.
Additional Information
© 1995 The American Physical Society. Received 30 January 1995. This work is supported by NSF Grant No. PHY-9408905. Partial support of NIH (Grant No. GM-50712) and HSFP is also acknowledged. We thank J. Howard for discussions and for providing us with purified kinesin, T.E. Holy for his contribution to the numerical simulation, and A. Ott for this contribution to the actin myosin experimental setup.Attached Files
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Additional details
- Eprint ID
- 8915
- Resolver ID
- CaltechAUTHORS:BOUprl95
- PHY-9408905
- NSF
- GM-50712
- NIH
- Human Frontier Science Program
- Created
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2007-09-26Created from EPrint's datestamp field
- Updated
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2021-11-08Created from EPrint's last_modified field