Isolation of a peptide in guinea pig liver homogenate and its turnover of leucine

Creators: Borsook, Henry; Deasy, Clara L.; Haagen-Smit, A. J.; Keighley, Geoffrey; Lowy, Peter H.

Abstract

Leucine was synthesized with C14 in the carboxyl group. 10 mg. of the radioactive amino acid (DL) and 0.66 gm. (wet weight) of guinea pig liver homogenate were added to a reaction mixture containing 1.3 per cent of an amino acid mixture corresponding to the composition of casein and 0.005 M fumarate, all in a final volume of 4 ml. of isotonic saline solution(1) at pH 7.4. The reaction was carried out under oxygen for 6 hours at 38°.

Additional Information

© 1948 American Society of Biological Chemists. Received for publication, May 14, 1948. The authors are indebted to and wish to thank Dr. S. Moore and Dr. W.H. Stein of The Rockefeller Institute for Medical Research for their generosity and courtesy in giving the details of their method of starch chromatography and ninhydrin calorimetry prior to publication. This work is part of that done under contract with the Office of Naval Research, United States Navy Department. The C14 used in this investigation was supplied by the Monsanto Chemical Company, Clinton Laboratories, and was obtained on allocation from the United States Atomic Energy Commission.

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