Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published July 1972 | Published
Journal Article Open

Direction of Translation and Size of Bacteriophage [var phi]X174 Cistrons

Abstract

Translation of the bacteriophage [var phi]X174 genome follows cistron order D-E-F-G-H-A-B-C. To establish this, the position of a nonsense mutation on the genetic map was compared with the physical size (molecular weight) of the appropriate protein fragment generated in nonpermissive cells. Distances on the [var phi]X174 genetic map and distances on a physical map constructed from the molecular weights of [var phi]X174 proteins and protein fragments are proportional over most of the genome with the exception of the high recombination region within cistron A.

Additional Information

© 1972, American Society for Microbiology Received for publication 20 March 1972 We thank Paul Howard-Flanders for the initial idea to study the genetic recombination of bacteriophage [phi]X174. The excellent technical assistance of Gloria Davis, Alma Shafer, David Holmes, and Jill Fabricant Hiatt is acknowledged. A preliminary account of these results was given at the Bacteriophage Meetings, Cold Spring Harbor, 27-31 August 1970. This research was carried out with the assistance of Public Health Service training grant GM-86 and research grant GM13554 from the National Institute of General Medical Sciences.

Attached Files

Published - BENjvir72.pdf

Files

BENjvir72.pdf
Files (2.4 MB)
Name Size Download all
md5:0897cb18d7b1eec95b26fd4167aa185e
2.4 MB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 23, 2023