Published April 1986
| public
Journal Article
Open
Lack of evidence for VPg priming of poliovirus RNA synthesis in the host factor-dependent in vitro replicase reaction
- Creators
- Andrews, Nancy C.
-
Baltimore, David
Abstract
Anti-VPg immunoprecipitable RNA labeled in vitro during a poliovirus RNA polymerase reaction was formed by the elongation of VPg-containing template fragments rather than by initiation with VPg. The reaction was dependent on a host factor (terminal uridylyl transferase). The incorporation of labeled UTP could be detected with only the host factor present.
Additional Information
Copyright © 1986 by the American Society for Microbiology. Received 30 September 1985/Accepted 25 November 1985 We thank M. Baron for anti-VPg antiserum. N.C.A. was partially supported by an M.S.T.P. training grant from the National Institute for General Medical Sciences (2T 32 GM07753-06); this work was also supported by Public Health Service grant A122346 from the National Institute of Allergy and Infectious Diseases to D.B.Files
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Additional details
- Eprint ID
- 2286
- Resolver ID
- CaltechAUTHORS:ANDjvir86
- Created
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2006-03-21Created from EPrint's datestamp field
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2019-10-02Created from EPrint's last_modified field