Welcome to the new version of CaltechAUTHORS. Login is currently restricted to library staff. If you notice any issues, please email coda@library.caltech.edu
Published August 1, 1971 | public
Journal Article Open

Symmetrical In Vivo transcription of mitochondrial DNA in HeLa cells

Abstract

RNA·DNA hybridization experiments utilizing separated strands of HeLa mitochondrial DNA and mit-RNA from HeLa cells exposed to short pulses of [5-3H]uridine have shown that the labeled RNA hybridizes with both the light (L) and the heavy (H) strand, though to a different relative extent depending upon the labeling time. Thus, hybridization of pulse-labeled RNA is about equal with the two strands when the pulse is very short (1-5 min), and becomes more and more predominant with the H strand with increasing pulse length. Pulse-labeled fast-sedimenting mit-RNA forms RNase-resistant double-stranded structures up to more than 5 µm long when self-annealed or annealed with an excess of unlabeled mit-RNA. These observations and the previous evidence of complete transcription of the H strand strongly suggest that mit-DNA is transcribed in HeLa cells symmetrically over a considerable portion of its length, with the transcript of the L strand being rapidly degraded or otherwise removed from the mitochondrial fraction.

Additional Information

Copyright © 1971 by the National Academy of Sciences. Communicated by Renato Dulbecco, May 25, 1971. These investigations were supported by a research grant from the U.S. Public Health Service (GM-11726) and by a Dernham Fellowship of the American Cancer Society to one of us (Y. A.). The help of Mrs. L. Wenzel and Mrs. B. Keeley is gratefully acknowledged. This is article VII in the series Expression of the Mitochondrial Genome in HeLa Cells. Paper VI is ref. 10.

Files

ALOpnas71.pdf
Files (1.4 MB)
Name Size Download all
md5:e9013ed420664c69e7602426f87bc82e
1.4 MB Preview Download

Additional details

Created:
August 22, 2023
Modified:
October 13, 2023