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Published March 1, 2005 | Published
Journal Article Open

Harmonic phase-dispersion microscope with a Mach-Zehnder interferometer

Abstract

Harmonic phase-dispersion microscopy (PDM) is a new imaging technique in which contrast is provided by differences in refractive index at two harmonically related wavelengths. We report a new configuration of the harmonic phase-dispersion microscope in a Mach-Zehnder geometry as an instrument for imaging biological samples. Several improvements on the earlier design are demonstrated, including a single-pass configuration and acousto-optic modulators for generating the heterodyne signals without mechanical arm scanning. We demonstrate quantitative phase-dispersion images of test structures and biological samples.

Additional Information

© 2005 Optical Society of America Received 3 June 2004; revised manuscript received 20 October 2004; accepted 20 October 2004. This research was supported by the National Institutes of Health grant P41-RR02594-18 and by the Hamamatsu Corporation.

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August 22, 2023
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