Published October 14, 2022 | Published
Journal Article Open

Plant biomacromolecule delivery methods in the 21st century

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Abstract

The 21st century witnessed a boom in plant genomics and gene characterization studies through RNA interference and site-directed mutagenesis. Specifically, the last 15 years marked a rapid increase in discovering and implementing different genome editing techniques. Methods to deliver gene editing reagents have also attempted to keep pace with the discovery and implementation of gene editing tools in plants. As a result, various transient/stable, quick/lengthy, expensive (requiring specialized equipment)/inexpensive, and versatile/specific (species, developmental stage, or tissue) methods were developed. A brief account of these methods with emphasis on recent developments is provided in this review article. Additionally, the strengths and limitations of each method are listed to allow the reader to select the most appropriate method for their specific studies. Finally, a perspective for future developments and needs in this research area is presented.

Additional Information

© 2022 Rustgi, Naveed, Windham, Zhang and Demirer. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. This work was supported by grants from Clemson University (Clemson SEED), National Institute of Food and Agriculture (NIFA Multi-state grant S009), SC Peanut Board, National Peanut Board, and Foundation for Food & Agriculture Research (FFAR) to SR. GD is supported by the Resnick Sustainability Institute at California Institute of Technology. HZ acknowledges the support from Natural Science Foundation of Guangdong Province of China (2022A1515011839). Author contributions. SR, SN, JW, HZ, and GD wrote the manuscript. GD designed the figure. SR contributed to the conception. All authors contributed to the revision, editing, and approval of the manuscript. The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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Created:
August 22, 2023
Modified:
October 20, 2023