Highly efficient and rapid generation of genetic variants

Creators: Liu, Liyuan; Wang, Harris H.; Wang, Kaihang; Mukhopadhyay, Madhura

Abstract

A new mutagenesis platform enables the fast, cost-efficient and automatable production of defined multi-site sequence variants for a wide range of applications. Demonstrations of this method included the generation of SARS-CoV-2 spike gene variants, DNA fragments for large-scale genome engineering, and adeno-associated virus 2 (AAV2) cap genes with improved packaging capacity.

Additional Information

© 2023 Springer Nature America, Inc. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. This is a summary of: Liu, L., Huang, Y. & Wang, H. H. Fast and efficient template-mediated synthesis of genetic variants. Nat. Methods https://doi.org/10.1038/s41592-023-01868-1 (2023)

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