Structure and Assembly of the Proteus mirabilis Flagellar Motor by Cryo-Electron Tomography
- Creators
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Kaplan, Mohammed
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Yao, Qing
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Jensen, Grant J.
Abstract
Proteus mirabilis is a Gram-negative Gammaproteobacterium and a major causative agent of urinary tract infections in humans. It is characterized by its ability to switch between swimming motility in liquid media and swarming on solid surfaces. Here, we used cryo-electron tomography and subtomogram averaging to reveal the structure of the flagellar motor of P. mirabilis at nanometer resolution in intact cells. We found that P. mirabilis has a motor that is structurally similar to those of Escherichia coli and Salmonella enterica, lacking the periplasmic elaborations that characterize other more specialized gammaproteobacterial motors. In addition, no density corresponding to stators was present in the subtomogram average suggesting that the stators are dynamic. Finally, several assembly intermediates of the motor were seen that support the inside-out assembly pathway.
Additional Information
© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). (This article belongs to the Special Issue Flagella 2.0) We thank Douglas Weibel at the University of Wisconsin–Madison for providing the Proteus mirabilis strain. We are grateful to George Auer from the University of Wisconsin–Madison for his help in preparing the swarmer cells. This project was funded by the National Institutes of Health (grant R01 AI127401 to G.J.J). Author Contributions. Conceptualization, M.K. and G.J.J.; methodology, M.K. and Q.Y.; software, M.K. and Q.Y.; validation, M.K., Q.Y. and G.J.J.; formal analysis, M.K. and G.J.J.; investigation, M.K.; resources, M.K. and G.J.J.; data curation, M.K. and Q.Y.; writing—original draft preparation, M.K.; writing—review and editing, M.K., Q.Y. and G.J.J.; visualization, M.K. and Q.Y.; supervision, G.J.J.; project administration, M.K. and G.J.J.; funding acquisition, G.J.J. All authors have read and agreed to the published version of the manuscript. Data Availability Statement. All tomograms are available upon request. The authors declare no conflict of interest.Attached Files
Published - ijms-24-08292.pdf
Supplemental Material - ijms-24-08292-s001.zip
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Additional details
- PMCID
- PMC10179241
- Eprint ID
- 121452
- Resolver ID
- CaltechAUTHORS:20230519-1595000.11
- R01 AI127401
- NIH
- Created
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2023-05-22Created from EPrint's datestamp field
- Updated
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2023-05-22Created from EPrint's last_modified field
- Caltech groups
- Division of Biology and Biological Engineering