The Role of 1-O-Acylceramide NP in Structural Organization and Permeability of the Stratum Corneum Lipid Matrix
Abstract
The extracellular lipid matrix in the stratum corneum (SC) is crucial for generating a skin barrier (permeability) function. The lipid matrix contains three major components; ceramides, cholesterol, and free fatty acids. The broad diversity of ceramides depends on their molecular structures (e.g., hydroxylations and chain lengths) and plays a critical role in maintaining the structural integrity of the lipid matrix. Although recent studies identified a new subclass of ceramide, 1-O-acylceramide NP (CerENP), its precise role in the lipid matrix of SC is still elusive. Herein, we investigate the role of CerENP on the structure and permeability of the SC by molecular dynamics simulations. Our results suggest that the CerENP molecules induce a denser lipid matrix in the lateral dimension in the long periodicity phase model with a bilayer-slab- bilayer structure. Moreover, ethanol permeability analysis indicates that CerENP can suppress molecular permeability through the lipid matrix. This study provides insight into the role of a new subclass of ceramide in the SC, which can lead to our better understanding of skin organization and disease-related barrier dysfunction.
Additional Information
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This research was supported by a grant from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI), funded by the Ministry of Health & Welfare, Republic of Korea (grant number: HP20C0018). Computations were supported by the National Institute of Supercomputing and Network/Korea Institute of Science and Technology Information with supercomputing resources including technical support (KSC-2018-S1-0004). The authors have declared no competing interest.Attached Files
Submitted - 2022.12.06.519381v1.full.pdf
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Additional details
- Eprint ID
- 120168
- Resolver ID
- CaltechAUTHORS:20230316-182748000.57
- HP20C0018
- Ministry of Health and Welfare (Korea)
- KSC-2018-S1-0004
- National Supercomputing Center (Korea)
- Created
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2023-03-17Created from EPrint's datestamp field
- Updated
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2023-03-17Created from EPrint's last_modified field