Raman Scattering Spectroscopy on Strawberry DNA
Abstract
Raman scattering spectroscopies are important analytical tools in modern chemistry. Here, we describe the construction of the unamplified Raman scattering analysis (URSA) instrument: an inexpensive, open-table Raman spectrometer that uses a 532 nm diode-pumped laser as a light source. Upon application of this spectrometer to DNA extracted from strawberries with a soap solution and precipitated in isopropanol, two major Raman peaks with shifts of 740 and 2970 cm⁻¹ were observed, consistent with previously published DNA Raman spectra. In this general article, we discuss the potential implementation of this work in an upper-level undergraduate laboratory, which would allow for hands-on exposure to both spectroscopic instrumental design and biochemical sample complexity.
Additional Information
The graphical abstract and Figure 1 were created with BioRender.com. This work was funded by the Los Alamos National Laboratory Strategic Investment Initiative of the Richard P. Feynman Center for Innovation. This work was performed at the Los Alamos National Laboratory, which is operated by Triad National Security, LLC, for the National Nuclear Security Administration of the U.S. Department of Energy (contract 89233218CNA000001).Additional details
- Eprint ID
- 117543
- Resolver ID
- CaltechAUTHORS:20221024-123751900.12
- Los Alamos National Laboratory
- 89233218CNA000001
- Department of Energy (DOE)
- Created
-
2022-10-28Created from EPrint's datestamp field
- Updated
-
2022-11-01Created from EPrint's last_modified field