Published April 13, 2022
| public
Book Section - Chapter
The Giant Axon of the Squid: A Simple System for Axonal Transport Studies
- Other:
- Vagnoni, Alessio
Abstract
The squid giant axon has a long history of being a superb experimental system in which to investigate a wide range of questions concerning intracellular transport. In this protocol we describe the method used for dissecting the axon to preserve its viability in vitro, and the technique for injecting exogenous materials into the living axon. Now that the squid genome is emerging, and the CRISPR/cas9 system has been successfully applied to knock-out squid genes, the giant axon will resume its place in the scientific pantheon of powerful experimental systems in which to address biological questions pertaining to all eukaryotes.
Additional Information
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022. First Online: 13 April 2022. We are grateful to Prasanna Satpute-Krishnan, Derek Nobrega, and Michael P. Conley for development of this protocol and some of the drawings, to Thomas S. Reese for inspiration and guidance, to Jim Galbraith for construction of the squid table, to Mark Terasaki and Laurinda Jaffe for injection advice, and to the Marine Biological Laboratory Embryology Course for loan of the micromanipulator and other injection equipment. We also thank Paulette Ferland for technical assistance. We co-authors were all at Brown University when this work was developed and we remain Brown Alumni. We are grateful to Brown University for the extraordinary opportunity afforded us to work together: Bearer as tenured professor and PI, DeGiorgis as PhD candidate, and Jang in the BA-MD program. This work was funded by Frederick Bang Summer Whitman Fellowships from MBL (E.L.B.), the Dart Foundation (E.L.B.) as well as NIGMS RO1 GM47368, NINDS RO1 NS046810 and RO1 NS062184 (E.L.B.), and the National Science Foundation under EPSCoR Cooperative Agreement #OIA-1655221 (J.A.D.).Additional details
- Eprint ID
- 114678
- Resolver ID
- CaltechAUTHORS:20220511-183651271
- Marine Biological Laboratory
- Dart Foundation
- RO1 GM47368
- NIH
- RO1 NS046810
- NIH
- RO1 NS062184
- NIH
- OIA-1655221
- NSF
- Created
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2022-05-11Created from EPrint's datestamp field
- Updated
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2022-05-11Created from EPrint's last_modified field
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 2431