Genetic Recombination in Sexual Crosses Of Phycomyces

Creators: Eslava, A. P.; Alvarez, M. I.; Burke, Patricia V.; Delbrück, M.

Abstract

Sexual crosses between strains of Phycomyces blakesleeanus, involving three auxotrophic and one color marker and yielding a high proportion of zygospore germination, are described. Samples of 20-40 germ spores from 311 individual fertile germ sporangia originating from five two-factor and three three-factor crosses were characterized. The results show: (1) absence of any contribution of apogamic nuclei to the progeny, (2) confirmation of Burgeff's conjecture that the germ spores of any germ sporangium in most cases derive from one meiosis. In a cross involving two allelic markers the analysis of 175 pooled germ sporangia suggests an intragenic recombination frequency of 0.6%. All other factor combinations tested are unlinked. The bulk of the germ spores are homokaryotic. However, a small portion (4%) are heterokaryotic with respect to mating type.

Additional Information

© Genetics 1975. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model). Received: 07 February 1975; Published: 28 July 1975. A. P. E. wishes to thank Comisión de Intercambio Cultural entre España y U.S.A. for financial support. We would further like to thank Jeanette Navest, Peggy Lockhart, and Doris Tsongas for their kind assistance. Part of this work was done during summer workshops in 1973 and 1974 generously supported by the Cold Spring Harbor Laboratory. This work was supported in part by the National Science Foundation (U.S.A.) grant BMS 70-00999 AO4.

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