Imaging Neuropeptide Release at Drosophila Neuromuscular Junction with a Genetically Engineered Neuropeptide Release Reporter
- Creators
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Han, Yifu
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Ding, Keke
- Others:
- Dahlmanns, Jana
- Dahlmanns, Marc
Abstract
Despite the important roles of neuropeptides in a variety of physiological processes, there still lacks a method to probe neuropeptide release events in vivo with satisfying temporal and spatial resolution. Neuropeptide Release Reporter (NPRR) was recently introduced as a novel genetically encoded indicator of neuropeptide release with a high temporal resolution and peptide specificity based on GCaMP molecule. Here we describe a method for using NPRR to image selective neuropeptide release at Drosophila neuromuscular junction in semi-dissected larvae. This method provides a quantitative analysis of activity-dependent neuropeptide release as real-time changes in fluorescence intensity of GCaMP reporter with sub-second temporal resolution and single bouton specificity.
Additional Information
© The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2022. Work in the Dickman lab is funded by a grant from the National Institutes of Health (NS091546). We thank Taylor Seid (California Institute of Technology), David Anderson (California Institute of Technology), and Dion Dickman (University of Southern California) for insightful discussions and comments. The authors declare no conflicts or other competing interests.Additional details
- Eprint ID
- 114286
- DOI
- 10.1007/978-1-0716-1916-2_15
- Resolver ID
- CaltechAUTHORS:20220413-42551000
- NS091546
- NIH
- Created
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2022-04-13Created from EPrint's datestamp field
- Updated
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2022-04-13Created from EPrint's last_modified field
- Series Name
- Methods in Molecular Biology
- Series Volume or Issue Number
- 2417