In situ architecture of human kinetochore-microtubule interface visualized by cryo-electron tomography
- Creators
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Zhao, Wei
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Jensen, Grant J.
Abstract
AbstractFaithful segregation of chromosomes during mitosis relies on a carefully coordinated and intricate interplay between the centromere, kinetochore, and spindle microtubules. Despite its importance, the architecture of this interface remains elusive. Here we used in situ cryo-electron tomography to visualize the native architecture of the kinetochore-microtubule interface in human U2OS cells at different stages of mitosis. We find that the centromere forms a pocket-like structure around kinetochore microtubules. Two morphologically distinct fibrillar densities form end-on and side-on connections to the plus-ends of microtubules within this centromeric pocket. Our data suggest a dynamic kinetochore-microtubule interface with multiple interactions between outer kinetochore components and spindle microtubules.
Additional Information
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC 4.0 International license. : We thank Dr. Songye Chen and Dr. Andrey Malyutin for technical assistance with cryo-electron microscopy. We thank Dr. Catherine M. Oikonomou for her very helpful comments on the manuscript. Cryo-electron microscopy was performed in the Beckman Institute Resource Center for Transmission Electron Microscopy at Caltech. This work was supported by NIH grant P50 AI150464 to G.J.J. Author contributions: W.Z. and G.J.J. conceived of the project. W.Z. and G.J.J. designed the experiments. W.Z. performed and interpreted the experiments. W.Z. and G.J.J. wrote the paper. Data and materials availability: All data is available upon request. Tomograms will be made publicly available through the Caltech Electron Tomography Database at https://etdb.caltech.edu. The authors have declared no competing interest.Attached Files
Submitted - 2022.02.17.480955v1.full.pdf
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Additional details
- Eprint ID
- 113519
- Resolver ID
- CaltechAUTHORS:20220222-705804000
- P50 AI150464
- NIH
- Created
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2022-02-24Created from EPrint's datestamp field
- Updated
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2022-02-24Created from EPrint's last_modified field
- Caltech groups
- Division of Biology and Biological Engineering