Putting MicroED to the Test: An Account of the Evaluation of 30 Diverse Pharmaceutical Compounds
Abstract
The application of microcrystal electron diffraction (microED) to a variety of pharmaceutical compounds is reported. The examples and work detailed showcase the utility of microED as a routine technique for the rapid collection, analysis, and generation of structural data on a number of pharmaceutically relevant compounds, requiring minimal sample preparation and often without the need for time-consuming vitrification and cryo transfer processes. The development of a scripted data processing workflow allowed for simultaneous collection and processing of electron diffraction data, further expediting structural analysis of fifteen compounds.
Additional Information
The content is available under CC BY NC 4.0 License. Financial support for this work was generously provided by the David and Lucile Packard Foundation (to H.M.N. & J.R.), the Arnold and Mabel Beckman Foundation (to J.R.) and the National Science Foundation (DGE-1650604 to J.E.B.) Author Contributions. H.M.N. and K.Q. conceived the idea and supervised the project. J.E.B. performed the described experiments and analyzed the data. The manuscript was written through contributions of all authors. J.E.B., H.M.N., J.R., and D.G. are founders of MicroEDLab.com, a for-profit contract research organization providing electron microscopy structural solutions.Attached Files
Submitted - putting-micro-ed-to-the-test-an-account-of-the-evaluation-of-30-diverse-pharmaceutical-compounds.pdf
Supplemental Material - supporting-information.pdf
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Additional details
- Eprint ID
- 111463
- Resolver ID
- CaltechAUTHORS:20211015-162628843
- David and Lucile Packard Foundation
- Arnold and Mabel Beckman Foundation
- NSF Graduate Research Fellowship
- DGE-1650604
- Created
-
2021-10-18Created from EPrint's datestamp field
- Updated
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2021-10-18Created from EPrint's last_modified field