Loss of the Bacterial Flagellar Motor Switch Complex upon Cell Lysis

Creators: Kaplan, Mohammed; Tocheva, Elitza I.; Briegel, Ariane; Dobro, Megan J.; Chang, Yi-Wei; Subramanian, Poorna; McDowall, Alasdair; Beeby, Morgan; Jensen, Grant J.

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Abstract

The bacterial flagellar motor is a complex macromolecular machine whose function and self-assembly present a fascinating puzzle for structural biologists. Here, we report that in diverse bacterial species, cell lysis leads to loss of the cytoplasmic switch complex and associated ATPase before other components of the motor. This loss may be prevented by the formation of a cytoplasmic vesicle around the complex. These observations suggest a relatively loose association of the switch complex with the rest of the flagellar machinery.

Additional Information

© 2021 Kaplan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received 5 February 2021; Accepted 26 April 2021; Published 8 June 2021. This project was funded by the NIH (grant RO1 AI127401 to G.J.J.) and a Baxter postdoctoral fellowship from Caltech to M.K. Cryo-ET work was done in the Beckman Institute Resource Center for Transmission Electron Microscopy at the California Institute of Technology. We are grateful to Catherine Oikonomou for critically reading the manuscript. We thank Cristina Iancu for collecting H. crunogenus data.

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