Published September 8, 2021
| Submitted
Report
Open
A novel widespread bacterial structure related to the flagellar type III secretion system
Abstract
The flagellar type III secretion system (fT3SS) is a suite of membrane-embedded and cytoplasmic proteins responsible for building the bacterial flagellar motility machinery. Homologous proteins form the injectisome machinery bacteria use to deliver effector proteins into eukaryotic cells, and other family members have recently been reported to be involved in the formation of membrane nanotubes. Here we describe a novel, ubiquitous and evolutionarily widespread hat-shaped structure embedded in the inner membrane of bacteria, of yet-unidentified function, that is related to the fT3SS, adding to the already rich repertoire of this family of nanomachines.
Additional Information
The copyright holder for this preprint is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under a CC-BY-NC-ND 4.0 International license. This version posted September 4, 2021. This project was funded by the NIH (grant R01 AI127401 to G.J.J., and P20 GM130456 and P30 GM110787 to C.L.S.) and a Baxter postdoctoral fellowship from Caltech to M.K. Cryo-ET work was done in the Beckman Institute Resource Center for Transmission Electron Microscopy at the California Institute of Technology. We are grateful to Prof. Marc Erhardt (Humboldt-Universitat zu Berlin) for critically reading an initial version of this work. We thank Prof. Elitza I. Tocheva for collecting the A. tumefaciens data, Dr. Jian Shi for collecting the H. neptunium data, and Prof. Martin Pilhofer for collecting the P. luteoviolacea data.Attached Files
Submitted - 2021.09.03.458937v1.full.pdf
Files
2021.09.03.458937v1.full.pdf
Files
(17.9 MB)
Name | Size | Download all |
---|---|---|
md5:afeb2b9054bed6c59c0822bfbc7e6f4a
|
17.9 MB | Preview Download |
Additional details
- Eprint ID
- 110754
- Resolver ID
- CaltechAUTHORS:20210907-221825591
- R01 AI127401
- NIH
- P20 GM130456
- NIH
- P30 GM110787
- NIH
- Baxter Foundation
- Created
-
2021-09-08Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field
- Caltech groups
- Division of Biology and Biological Engineering