Published March 15, 2010
| Published + Supplemental Material
Journal Article
Open
Label-free imaging of heme proteins with two-photon excited photothermal lens microscopy
Chicago
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Abstract
Heme proteins, such as hemoglobins and cytochromes, play important roles in various biological processes. Here we employ the two-photon excited photothermal effect as a contrast mechanism to map heme proteins distribution. Particularly, both a thermal lens scheme and a high-frequency modulation are utilized to enhance the signal-to-noise ratio. We demonstrate label-free imaging of individual red blood cells, subcellular distribution of cytochromes in live mammalian cells, and the microvascular networks in mouse ear tissue and in a zebrafish gill.
Additional Information
© 2010 American Institute of Physics. Received 21 November 2009; accepted 11 January 2010; published online 17 March 2010. We thank Christian Freudiger and Brian Saar for helpful discussions; Harvard MCB Mouse facility for providing euthanized mice, and Harvard MCB zebrafish facility for providing euthanized zebrafish. S.L. and W.M. contribute equally to this work. This work was supported by National Science Foundation (Grant No. DBI-0649892) and U.S Department of Energy's Basic Energy Sciences Program (Grant No. DE-FG02-07ER15875).Attached Files
Published - 1.3308485.pdf
Supplemental Material - supporting_materials_photothermal.doc
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Additional details
- Eprint ID
- 110708
- Resolver ID
- CaltechAUTHORS:20210902-233956011
- NSF
- DBI-0649892
- Department of Energy (DOE)
- DE-FG02-07ER15875
- Created
-
2021-09-07Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field