Plant PIEZO homologs modulate vacuole morphology during tip growth
Abstract
In animals, PIEZOs are plasma membraneālocalized cation channels involved in diverse mechanosensory processes. We investigated PIEZO function in tip-growing cells in the moss Physcomitrium patens and the flowering plant Arabidopsis thaliana. PpPIEZO1 and PpPIEZO2 redundantly contribute to the normal growth, size, and cytoplasmic calcium oscillations of caulonemal cells. Both PpPIEZO1 and PpPIEZO2 localized to vacuolar membranes. Loss-of-function, gain-of-function, and overexpression mutants revealed that moss PIEZO homologs promote increased complexity of vacuolar membranes through tubulation, internalization, and/or fission. Arabidopsis PIEZO1 also localized to the tonoplast and is required for vacuole tubulation in the tips of pollen tubes. We propose that in plant cells the tonoplast has more freedom of movement than the plasma membrane, making it a more effective location for mechanosensory proteins.
Additional Information
Ā© 2021 American Association for the Advancement of Science. This is an article distributed under the terms of the Science Journals Default License. Received 3 September 2020; resubmitted 24 February 2021. Accepted 16 June 2021. We thank S.-Z. Wu for generating the Vam3-GFP vacuolar marker, S. Ryken for help with calcium imaging, S.A.R. Mousavi and A. Patapoutian for codon-optimized AtPIEZO1 and helpful discussions, and E. Meyerowitz for the Arabidopsis thaliana CRISPR lines. E.S.H. received HHMI-Simons Faculty Scholar Grant 55108530, NSF MCB-1929355, and funding from the NSF Center for Engineering Mechanobiology. M.B. received NSF MCB-1330171. Author contributions: I.R., R.A.R., J.H.C., E.R.W., M.B., and E.S.H. designed and performed the experiments and analyzed the data. I.R., M.B., and E.S.H. wrote the paper. T.L. and C.S.B. created some of the reagents. The authors declare no competing interests. Data and materials availability: All data are available in the manuscript and supplementary materials.Attached Files
Supplemental Material - abe6310_MDAR_Reproducibility_Checklist.pdf
Supplemental Material - abe6310_Radin_SM.pdf
Supplemental Material - abe6310_TablesS1andS3.zip
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Additional details
- Eprint ID
- 110116
- Resolver ID
- CaltechAUTHORS:20210802-210424813
- 55108530
- Howard Hughes Medical Institute (HHMI)
- Simons Foundation
- MCB-1929355
- NSF
- MCB-1330171
- NSF
- Created
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2021-08-02Created from EPrint's datestamp field
- Updated
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2021-08-02Created from EPrint's last_modified field