Capillary electrophoresis method for analysis of inorganic and organic anions related to habitability and the search for life
Abstract
In situ missions of exploration require analytical methods that are capable of detecting a wide range of molecular targets in complex matrices without a priori assumptions of sample composition. Furthermore, these methods should also minimize the number of reagents needed and any sample preparation steps. We have developed a method for the detection of metabolically relevant inorganic and organic anions that is suitable for implementation on in situ spaceflight missions. Using 55 mM acetic acid, 50 mM triethylamine, and 5% glycerol, more than 21 relevant anions are separated in less than 20 minutes. The method is robust to sample ionic strength, tolerating high concentrations of background salts (up to 900 mM NaCl and 300 mM MgSO₄). This is an important feature for future missions to ocean worlds. The method was validated using a culture of Escherichia coli and with high salinity natural samples collected from Mono Lake, California.
Additional Information
© 2021 Wiley-VCH GmbH. Issue Online: 05 October 2021; Version of Record online: 01 August 2021; Accepted manuscript online: 20 July 2021; Manuscript accepted: 09 July 2021; Manuscript revised: 07 July 2021; Manuscript received: 06 May 2021. Research Funding: National Aeronautics and Space Administration. Data availability statement: The data that support the findings of this study are available from the corresponding author upon reasonable request.Attached Files
Supplemental Material - elps7469-sup-0001-suppmat.pdf
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Additional details
- Eprint ID
- 110083
- Resolver ID
- CaltechAUTHORS:20210729-215351588
- NASA
- Created
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2021-08-02Created from EPrint's datestamp field
- Updated
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2021-10-13Created from EPrint's last_modified field