Published July 9, 2021
| Published
Journal Article
Open
A Mutation of lips-6 in C. elegans
Abstract
The efficient CRISPR/Cas9 genome editing toolkit in the nematode Caenorhabditis elegans has made the creation of null mutants easier than ever. The putative lipid hydrolase lips-6 is known to function in the DAF-12/DIN-1 pathway to promote fat mobilization during periods of starvation in adult worms (Tao et al. 2016). Similarly, it was found to be strongly downregulated in response to dauer ascarosides given during the L1 to L2d decision in larval worms (Cohen et al. 2021). Here, we have created a mutant of the putative lipase lips-6 and studied its lifespan as well as its ascaroside profile.
Additional Information
© 2021 by the authors. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International (CC BY 4.0) License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. History: Received June 9, 2021 Revision received July 3, 2021 Accepted July 5, 2021 Published July 9, 2021. We thank Heenam Park and Frank Schroeder for their helpful discussion and comments. This material is based upon work supported by the National Science Foundation Graduate Research Fellowship under Grant No. DGE 1745301 to SMC and Grant No. R24OD023041 to PWS. Author Contributions: Sarah M Cohen: Conceptualization, Investigation, Methodology, Writing - original draft, Visualization. Henry H Le: Methodology, Visualization, Writing - review and editing, Investigation. Paul W Sternberg: Funding acquisition, Project administration, Resources, Supervision, Writing - review and editing. Reviewed By: Jennifer Watts.Attached Files
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Additional details
- PMCID
- PMC8271349
- Eprint ID
- 109818
- Resolver ID
- CaltechAUTHORS:20210714-172910613
- NSF Graduate Research Fellowship
- DGE-1745301
- NIH
- R24OD023041
- Created
-
2021-07-14Created from EPrint's datestamp field
- Updated
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2021-07-14Created from EPrint's last_modified field
- Caltech groups
- WormBase, Division of Biology and Biological Engineering (BBE)