Transcriptome-Scale Super-Resolved Imaging in Tissues by RNA SeqFISH
- Creators
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Cai, L.
Abstract
Imaging the transcriptome in situ with high accuracy has been a major challenge in single cell biology, particularly hindered by the limits of optical resolution and the density of transcripts in single cells. We developed seqFISH+, that can image the mRNAs for 10,000 genes in single cells with high accuracy and sub-diffraction-limit resolution, in the mouse brain cortex, subventricular zone, and the olfactory bulb, using a standard confocal microscope. The transcriptome level profiling of seqFISH+ allows unbiased identification of cell classes and their spatial organization in tissues. In addition, seqFISH+ reveals subcellular mRNA localization patterns in cells and ligand-receptor pairs across neighboring cells. This technology demonstrates the ability to generate spatial cell atlases and to perform discovery-driven studies of biological processes in situ.
Additional Information
© 2020 Nature Publishing Group. Published 01 December 2020.Additional details
- Eprint ID
- 107608
- Resolver ID
- CaltechAUTHORS:20210120-154339392
- Created
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2021-01-21Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field
- Caltech groups
- Division of Biology and Biological Engineering (BBE)