Imaging the Beating Heart with Macroscopic Phase Stamping
Abstract
We present a novel approach for imaging the beating embryonic heart, based on combining two independent imaging channels to capture the full spatio-temporal information of the moving 3D structure. High-resolution, optically-sectioned image recording is accompanied by simultaneous acquisition of low-resolution, whole-heart recording, allowing the latter to be used in post-acquisition processing to determine the macroscopic spatio-temporal phase of the heart beating cycle. Once determined, or 'stamped', the phase information common to both imaging channels is used to reconstruct the 3D beating heart. We demonstrated our approach in imaging the beating heart of the zebrafish embryo, capturing the entire heart over its full beating cycle, and characterizing cellular dynamic behavior with sub-cellular resolution.
Additional Information
Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0). We thank Leigh Ann Fletcher and Lorna McFarlane for fish care. This work was funded by the Rosen Center for Bioengineering (Caltech), a Center of Excellence in Genomic Science grant (NIH/NHGRI P50HR004071), a FaceBase grant (NIH Grant U01DE020063) and the Translational Imaging Center at USC.Attached Files
Submitted - 2011.01688.pdf
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Additional details
- Eprint ID
- 107407
- Resolver ID
- CaltechAUTHORS:20210111-160848952
- Donna and Benjamin M. Rosen Bioengineering Center
- NIH
- P50HR004071
- NIH
- U01DE020063
- University of Southern California
- Created
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2021-01-12Created from EPrint's datestamp field
- Updated
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2023-06-02Created from EPrint's last_modified field
- Caltech groups
- Rosen Bioengineering Center