Published September 24, 1971
| public
Journal Article
Collapse of extended deoxyribonucleoprotein molecules upon increase of the ionic strength of solution
Abstract
The increase of the ionic strength of extremely dilute deoxyribonucleo protein (DNP) solution from 0.001 to 0.1 results in an intramolecular structural transition, which is reflected by a 2.5-fold increase of the sedimentation coefficient of the DNP and by the electron microscopic appearance of DNP molecules. The rate of this structural transition is considerably higher at 37° than at 0°. Both sedimentation and electron microscopic data allow one to suggest the existence of a supercoiled DNA in collapsed DNP molecules. Removal of the very lysine-rich histone (F1) and of some nonhistone proteins from the DNP results in the loss of its ability to undergo the above structural transition.
Additional Information
© 1971 Published by Elsevier. (Received July 30th, 1971) We are greatly indebted to Professor G.P. Georgiev for support and discussions throughout this work. Thanks are also due to Drs. A.S. Tichonenko and N.E. Gluhova for advice on electron microscopy and to Dr. U.N. Mickelsaar for collaboration in the early stages of this work.Additional details
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