Telomere-to-Telomere Genome Assembly of Bursaphelenchus okinawaensis Strain SH1

Creators: Sun, Simo; Shinya, Ryoji; Dayi, Mehmet; Yoshida, Akemi; Sternberg, Paul W.; Kikuchi, Taisei

Abstract

Bursaphelenchus okinawaensis is a self-fertilizing, hermaphroditic, fungus-feeding nematode used as a laboratory model for the genus Bursaphelenchus, which includes the important pathogen Bursaphelenchus xylophilus. Here, we report the nearly complete genome sequence of B. okinawaensis. The 70-Mbp assembly contained six scaffolds (>11 Mbp each) with telomere repeats on their ends, indicating complete chromosomes.

Additional Information

© 2020 Sun et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Received 7 September 2020. Accepted 25 September 2020. Published 22 October 2020. We thank Asuka Konosu, Melis Konno, Ryusei Tanaka, Igor Antoschechkin, and the Jacobs Genome Facility for their technical assistance. This work was funded in part by Japan Society for the Promotion of Science (JSPS) KAKENHI grant numbers 26460510 and 19H03212 and JST CREST grant number JPMJCR18S7. Data availability: The B. okinawaensis v2 assembly has been deposited in DDBJ/EMBL/GenBank under BioProject number PRJEB40023. The raw Illumina, Nanopore, and Hi-C read data are available in the Sequence Read Archive with accession numbers DRR243691, DRR243689, and DRR243690, respectively.

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