Rat brain 5-HT_(1C) receptors are encoded by a 5-6 kbase mRNA size class and are functionally expressed in injected Xenopus oocytes
Abstract
Injection of rat brain RNA into Xenopus laevis oocytes induces synthesis of receptors that show an electrophysiological response to bath application of serotonin. While there are at least 4 pharmacologically distinct subtypes of 5-HT binding sites in the rat brain, we find that the pharmacological characteristics of the predominant electrophysiologically active receptor synthesized in Xenopus oocytes are most consistent with those of the 5-HT_(1C) subtype. Additional electrophysiologically active 5-HT receptor types could not be detected. Injection of mRNA isolated from a number of rat brain regions shows that the choroid plexus is particularly enriched for 5-HT_(1C) mRNA. Oocytes injected with RNA isolated from this region respond 16 or 8 times more strongly to serotonin than do oocytes injected with RNA isolated from cortex or substantia nigra, respectively. In addition, by fractionation of rat brain mRNA through agarose gels, we have identified a single RNA size class of about 5–6 kbase that encodes this serotonin receptor.
Additional Information
© 1987 by Society for Neuroscience. Beginning six months after publication the Work will be made freely available to the public on SfN's website to copy, distribute, or display under a Creative Commons Attribution 4.0 International (CC BY 4.0) license (https://creativecommons.org/licenses/by/4.0/). Received June 30, 1986; revised Oct. 16, 1986; accepted Oct. 17, 1986. The authors wish to thank Stephen J. Peroutka, Terry T. Takahashi, and Mary B. Kennedy for advice in receptor pharmacology and brain anatomy. We are grateful to Stephen J. Peroutka for donating RU 24969 and mesulergine, to Paul R. Hartig for supplying ketanserin, and to Beth J. Hoffman for critically reading the manuscript. This work was supported by NIH Grants GM-10991 and NS-11756 and by fellowships from the Deutsche Forschungsgemeinschaft to H.L., from the American Heart Association, Greater Los Angeles Affiliate, and NSERC of Canada to T.P.S., and from the Bantrell and Fulbright Foundations to N.D.Attached Files
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Additional details
- PMCID
- PMC6569006
- Eprint ID
- 106040
- Resolver ID
- CaltechAUTHORS:20201013-145241211
- NIH
- GM-10991
- NIH
- NS-11756
- Deutsche Forschungsgemeinschaft (DFG)
- American Heart Association, Greater Los Angeles Affiliate
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- Fulbright Foundation
- Created
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2020-10-13Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field