Inhibition of Polo kinase by BI2536 affects centriole separation during Drosophila male meiosis
Abstract
Pharmacological inhibition of Drosophila Polo kinase with BI2536 has allowed us to re-examine the requirements for Polo during Drosophila male gametogenesis. BI2536-treated spermatocytes persisted in a pro-metaphase state without dividing and had condensed chromosomes that did not separate. Centrosomes failed to recruit γ-tubulin and centrosomin (Cnn) and were not associated with microtubule arrays that were abnormal and did not form proper bipolar spindles. Centrioles, which usually separate during the anaphase of the first meiosis, remained held together in a V-shaped configuration suggesting that Polo kinase regulates the proteolysis that breaks centriole linkage to ensure their disengagement. Despite these defects spermatid differentiation proceeds, leading to axoneme formation.
Additional Information
© 2014 Landes Bioscience. Received 21 Mar 2014, Accepted 30 Apr 2014, Published online: 06 May 2014. The authors would like to thank Eyal Schejter and Timothy Megraw for generously providing antibodies and stocks. This work was supported by a grant from PRIN2012 to G.C. No potential conflicts of interest were disclosed.Attached Files
Supplemental Material - kccy_a_10929083_sm0001.zip
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Additional details
- PMCID
- PMC4111698
- Eprint ID
- 106026
- DOI
- 10.4161/cc.29083
- Resolver ID
- CaltechAUTHORS:20201013-122118067
- Created
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2020-10-13Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field