Dodeca satellite: a conserved G+C-rich satellite from the centromeric heterochromatin of Drosophila melanogaster

Creators: Abad, José P.; Carmena, Mar; Baars, Sigrid; Saunders, Robert D. C.; Glover, David M.; Ludeña, Paloma; Sentis, Carlos; Tyler-Smith, Chris; Villasante, Alfredo

Abstract

To identify sequences from the centromeric region, we have constructed a Drosophila melanogaster yeast artificial chromosome (YAC) library and screened it with purified DNA from the minichromosome Dp(1;f)1187 derived from the X chromosome. We describe the structure of one clone isolated in this way. This YAC is structurally unstable and contains tandemly repeated G+C-rich 11-mer and 12-mer units, which we call dodeca satellite. Most of this satellite is located near the centromere of an autosome. Cross-hybridizing sequences are found in the genomes of organisms as distant as Arabidopsis thaliana and Homo sapiens.

Additional Information

© 1992 National Academy of Sciences. Communicated by Antonio García-Bellido, February 3, 1992 (received for review December 11, 1991) We wish to thank J. Modolell and S. Campuzano for the gift of the Asc94 clone, R. Gonzalez-Duarte for supplying several Drosophila species, J. M. Martinez-Zapater for Arabidopsis DNA, L. Taylor for mammalian cell culture, and C. González for his generous help. We also thank E. Southern for comments on the manuscript. We give special thanks to J. Ortin and P. Ripoll for their constant support. This work was supported by the Dirección General de Investigación Científica y Técnica, the Science EC programme, and the Fundación R. Areces. The sequences reported in this paper have been deposited in the GenBank data base (accession nos. M86306-M86309). The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

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