Published March 12, 1999
| public
Journal Article
Abnormal Spindle Protein, Asp, and the Integrity of Mitotic Centrosomal Microtubule Organizing Centers
- Creators
- do Carmo Avides, Maria
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Glover, David M.
Abstract
The product of the abnormal spindle (asp) gene was found to be an asymmetrically localized component of the centrosome during mitosis, required to focus the poles of the mitotic spindle in vivo. Removing Asp protein function from Drosophila melanogaster embryo extracts, either by mutation or immunodepletion, resulted in loss of their ability to restore microtubule-organizing center activity to salt-stripped centrosome preparations. This was corrected by addition of purified Asp protein. Thus, Asp appears to hold together the microtubule-nucleating γ-tubulin ring complexes that organize the mitotic centrosome.
Additional Information
© 1999 American Association for the Advancement of Science. Received for publication November 19, 1998. Accepted for publication January 28, 1999. We are grateful to P. Ripoll for stimulating our interest in asp through the original observations of his laboratory and for his continued encouragement. We thank A. Desai, M. Moritz, and B. Lange for their valuable advice about protocols for centrosome purification and microtubule nucleation. We are grateful to L. Goldstein for the antibody to KLP61F. M.C.A. held an European Molecular Biology Organization fellowship during the initial stages of this work, which was supported by grants from the International Association of Cancer Research and Cancer Research Campaign of Great Britain.Additional details
- Eprint ID
- 105825
- DOI
- 10.1126/science.283.5408.1733
- Resolver ID
- CaltechAUTHORS:20201005-153617168
- European Molecular Biology Organization (EMBO)
- International Association of Cancer Research
- Cancer Research Campaign
- Created
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2020-10-06Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field