Gene amplification in Rhynchosciara salivary gland chromosomes
Abstract
Late in the fourth larval instar, several regions of the Rhynchosciara americana salivary gland chromosomes undergo "DNA puffing." We have constructed a library of cloned cDNAs synthesized from poly(A)⁺RNA isolated from salivary glands during the period of development when the DNA puffs are active. From this library we have studied clones representative of three genes active during this period but not active at earlier developmental periods of the gland. One of these genes is not amplified during the developmental process and encodes a 0.6-kilobase RNA molecule. The other two genes are located within the DNA-puff sites C3 and C8 and encode 1.25-kilobase and 1.95-kilobase RNA molecules, respectively. We estimate from the quantitation of transfer hybridization experiments that each of these genes undergoes 16-fold amplification during DNA puffing.
Additional Information
© 1982 National Academy of Sciences. Communicated by Joseph G. Gall, February 1, 1982. We thank Mike Scott, Debbie Smith, and Mike Lovett for technical advice; Prof. W. Beermann for his continued support; and Claus Pelling, Cor Hollenberg, Werner Büsen, Orilio Leoncini, and Manuel Diaz for interesting discussions. This work was principally supported by a grant from the Stiftung Volkswagenwerk and also by grants from the Fundagio de Amparo a Pesquisa do Estado de Sao Paulo and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). D.M.G. held a fellowship under the exchange program of the Royal Society and the CNPq. The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U. S. C. §1734 solely to indicate this fact.Attached Files
Published - 2947.full.pdf
Files
| Name | Size | Download all |
|---|---|---|
|
md5:dfcbd05dbfa4690625cd937f04218e46
|
2.4 MB | Preview Download |
Additional details
- PMCID
- PMC346325
- Eprint ID
- 105816
- Resolver ID
- CaltechAUTHORS:20201005-143650238
- Stiftung Volkswagenwerk
- Fundação de Amparo à Pesquisa do Estado de Sao Paulo (FAPESP)
- Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)
- Royal Society
- Created
-
2020-10-05Created from EPrint's datestamp field
- Updated
-
2021-11-16Created from EPrint's last_modified field