Two distinct mechanisms localise cyclin B transcripts in syncytial Drosophila embryos
Abstract
We demonstrate that two independent mechanisms act on maternally derived cyclin B transcripts to concentrate the transcripts at the posterior pole of the Drosophila oocyte and at the cortex of the syncytial embryo. The cortical accumulation occurs because the cyclin B transcript is concentrated around nuclei and comigrates with them to the cortex. The perinuclear localisation of the transcript is blocked by inhibitors of microtubule polymerisation and the transcript colocalises with microtubular structures during the cell cycle, suggesting that the transcript is associated either directly or indirectly with microtubules. Neither microtubules nor actin filaments are required to maintain the posterior concentration of cyclin B transcripts. Instead, this seems to depend on the association of the transcripts with a component of the posterior cytoplasm. The distribution pattern of the transcript at the posterior pole throughout embryogenesis and in a variety of mutant embryos suggests that this component is associated with polar granules.
Additional Information
© 1990 by Company of Biologists. Accepted 7 September 1990. We thank Paul Lasko for generously providing the vasa antibody used in this study and for communicating results prior to publication. The mutant fly stocks were kindly donated by C. Nüsslein-Volhard and P. Lasko. We also thank Charles Girdham and Salud Llamazares for providing constructs.Attached Files
Published - 1249.full.pdf
Files
Name | Size | Download all |
---|---|---|
md5:6ba3852f3671b52480d2a02943f11e60
|
8.3 MB | Preview Download |
Additional details
- Eprint ID
- 105810
- Resolver ID
- CaltechAUTHORS:20201005-143626240
- Created
-
2020-10-05Created from EPrint's datestamp field
- Updated
-
2020-10-05Created from EPrint's last_modified field