Revisiting the Role of the Mother Centriole in Centriole Biogenesis
Abstract
Centrioles duplicate once in each cell division cycle through so-called templated or canonical duplication. SAK, also called PLK4 (SAK/PLK4), a kinase implicated in tumor development, is an upstream regulator of canonical biogenesis necessary for centriole formation. We found that overexpression of SAK/PLK4 could induce amplification of centrioles in Drosophila embryos and their de novo formation in unfertilized eggs. Both processes required the activity of DSAS-6 and DSAS-4, two molecules required for canonical duplication. Thus, centriole biogenesis is a template-free self-assembly process triggered and regulated by molecules that ordinarily associate with the existing centriole. The mother centriole is not a bona fide template but a platform for a set of regulatory molecules that catalyzes and regulates daughter centriole assembly.
Additional Information
© 2007 American Association for the Advancement of Science. Received for publication March 23, 2007. Accepted for publication April 17, 2007. We thank C. Ferreira and I. Ferreira for help with experiments; D. Johnston, R. Basto, and J. Raff for reagents; R. Martinho, J. Leal, M. Gatt, R. Kuriyama, and anonymous reviewers for comments on the manuscript; the Drosophila Instituto Gulbenkian de Ciência (IGC) community, G. Goshima, and the M.B.-D. and D.M.G. groups for discussions; thebestgene.com for making transgenic flies; and the IGC imaging unit for help with image acquisition. We are grateful for grants from Cancer Research UK, Fundação Calouste Gulbenkian, and Fundação para a Ciência e Tecnologia (POCI2010) and for an International Joint Project grant from the Royal Society for collaboration between the M.B.-D. and D.M.G. groups.Attached Files
Supplemental Material - Rodrigues-Martins.SOM.pdf
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Additional details
- Eprint ID
- 105758
- DOI
- 10.1126/science.1142950
- Resolver ID
- CaltechAUTHORS:20201002-140639487
- Cancer Research UK
- Fundação Calouste Gulbenkian
- Fundação para a Ciência e Tecnologia (FCT)
- POCI2010
- Royal Society
- Created
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2020-10-05Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field