Abnormal anaphase resolution (aar): a locus required for progression through mitosis in Drosophila

Creators: Gomes, R.; Karess, R. E.; Ohkura, H.; Glover, D. M.; Sunkel, C. E.

Abstract

We describe a new mitotic locus of Drosophila melanogaster required for the progression through mitosis in the syncytial embryo and in late larval development. The locus aar (abnormal anaphase resolution) maps to the cytological interval 85E7-F16 and was identified by two alleles. The aar¹ allele causes pupal lethality. Larval neuroblasts show an elevated mitotic index with high chromosome condensation and stretched and lagging chromatids during anaphase. aar² produces fully viable but sterile females. aar¹/aar² females lay eggs that develop mitotic figures with similar abnormalities to those observed in neuroblasts. Indirect immunofluorescence of these embryos indicates that the centrosome cycle appears normal, although some abnormal spindle microtubules can be seen during mitosis.

Additional Information

© 1993 by Company of Biologists. (Received 25 August 1992 - Accepted 13 October 1992) The initial stages of this work were carried out in Imperial College, London, with the support of a Medical Research Council Grant to D.M.G. and R.E.K. Subsequent work in the UK was supported by grants from the Cancer Research Campaign and the Human Frontiers Science Programme to D.M.G. and H.O. The work in Porto was supported by a Grant from the Junta Nacional de Investigação Científica e Tecnológica (PMCT) to CES and the Instituto Nacional de Investigação Científica. We thank Isabel Salazar for immunofluorescence preparations and Adelaide Santos for general technical assistance.

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