Mutation of a gene for a Drosophila kinesin-like protein, Klp38B, leads to failure of cytokinesis
Abstract
Mutations in a gene (Klp38B) encoding a novel kinesin-like protein in Drosophila melanogaster lead to the formation of polyploid cells in the larval central nervous system and in the follicle cells of adult egg chambers. Some homozygous mutants survive to adulthood and also exhibit morphological defects indicative of abnormal cell cycle progression, including rough eyes, missing bristles, and abnormal abdominal cuticles. In larval brains, there is no accumulation of mitotic cells and the frequency of anaphase figures is comparable to wild type, suggesting that nuclear division is not affected. Such brains contain polyploid cells with metaphase and anaphase chromosomes associated with bipolar spindles. Such spindles have a number of unseparated centrosomes at their poles reflecting the degree of polyploidy of the cell. Follicle cells frequently contain two nuclei of roughly equal size. Taken together, we conclude that these Klp38B mutations lead to a failure of cytokinesis resulting in polyploidy, and discuss whether or not this is a direct effect of the mutation.
Additional Information
© 1997 by Company of Biologists. (Received 6 September 1996 – Accepted, in revised form, 4 February 1997) This work was supported by grants from the Cancer Research Campaign to Dundee, and the European Union Human Capital and Mobility and PECO Programmes to Dundee and Szeged. I.K. is also grateful for support from the National Foundation for Scientific Research in Hungary (OTKA no. 924). We thank Pedro Ripoll, Luke Alphey and Yoshihiro Inoue for discussions and communication of their results prior to publication. We thank Nick Brown, Inga Sidén-Kiamos, Dan Branton, and Lisa Frenz and Mike Goldberg for their respective gifts of the cDNA library, cosmid clones, anti-spectrin antibody and staged poly(A)+ RNA. We also thank Fiona Cullen for sequencing.Attached Files
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Additional details
- Eprint ID
- 105697
- Resolver ID
- CaltechAUTHORS:20200930-144643398
- Cancer Research Campaign
- European Union
- 924
- Hungarian Scientific Research Fund (OTKA)
- Created
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2020-10-01Created from EPrint's datestamp field
- Updated
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2020-10-01Created from EPrint's last_modified field