5.8 S and 2 S rDNA is located in the 'transcribed spacer' region between the 18 S and 26 S rRNA genes in Drosophila melanogaster
- Creators
- Jordan, Bertrand R.
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Glover, David M.
Abstract
In Drosophila melanogaster, two small RNA species are found hydrogen bonded to 26 S rRNA:5.8 S rRNA, approximately 125 nucleotides long, and 2 S rRNA, 30 nucleotides long [1]. Evidence from pulse-chase experiments shows that they arise as the result of post transcriptional processing of 26 S RNA, and that the corresponding cleavages occur in the cytoplasm after the central cleavage of the 26 S molecule which occurs in the nucleus [1]. These results do not define completely the architecture of the 26 S molecule and a number of different models remain possible (fig.1). If it is assumed, by analogy with Xenopus laevis [2] that 5.8 S rRNA is coded by a DNA region located between the genes for 18 S and 26 S rRNAs, then class (c) models can be excluded. The experiments reported here examine this question directly by hybridization of ³²P-1abelled 5.8 S and 2 S RNAs to cloned D. melanogaster rDNA fragments [3].
Additional Information
© 1977 Federation of European Biochemical Societies. Received 1 April 1977. This work was supported by a Medical Research Council grant to D.M.G. and by grants from the Commissariat à l'Energie Atomique and the Centre National de la Recherche Scientifique to B.R.J. B.R.J. is also grateful for a short term EMBO fellowship which enabled him to work at Imperial College, London.Additional details
- Eprint ID
- 105618
- DOI
- 10.1016/0014-5793(77)80321-9
- Resolver ID
- CaltechAUTHORS:20200928-150626940
- Medical Research Council (UK)
- Commissariat à l'Energie Atomique (CEA)
- Centre National de la Recherche Scientifique (CNRS)
- European Molecular Biology Organization (EMBO)
- Created
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2020-09-28Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field