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Published March 20, 2012 | Supplemental Material
Journal Article Open

Klp10A, a Microtubule-Depolymerizing Kinesin-13, Cooperates with CP110 to Control Drosophila Centriole Length

Abstract

Klp10A is a kinesin-13 of Drosophila melanogaster that depolymerizes cytoplasmic microtubules [1]. In interphase, it promotes microtubule catastrophe [2, 3, 4]; in mitosis, it contributes to anaphase chromosome movement by enabling tubulin flux [1, 5]. Here we show that Klp10A also acts as a microtubule depolymerase on centriolar microtubules to regulate centriole length. Thus, in both cultured cell lines and the testes, absence of Klp10A leads to longer centrioles that show incomplete 9-fold symmetry at their ends. These structures and associated pericentriolar material undergo fragmentation. We also show that in contrast to mammalian cells where depletion of CP110 leads to centriole elongation [6], in Drosophila cells it results in centriole length diminution that is overcome by codepletion of Klp10A to give longer centrioles than usual. We discuss how loss of centriole capping by CP110 might have different consequences for centriole length in mammalian [6, 7, 8] and insect cells and also relate these findings to the functional interactions between mammalian CP110 and another kinesin-13, Kif24, that in mammalian cells regulates cilium formation.

Additional Information

© 2012 Elsevier. Under an Elsevier user license. Received 10 June 2011, Revised 6 December 2011, Accepted 24 January 2012, Available online 23 February 2012. We thank M. Bettencourt-Dias for the Spd2 entry clone construct; M. Savoian for dsRNA against Klp59C, 59D, and 67A; N.S. Dzhindzhev for the MTp-GFP-Spd2-expressing cell line; J. Raff for the Dplp antibody; T. Hays for the dynein antibody; and C. Janke for the GT335 antibody. We are grateful to Cancer Research UK for a program grant that supported this work. H.R.'s research was supported by a Marie Curie Intra-European Fellowship with the Sixth European Community Framework Programme and a European Postdoctoral Fellowship from Fondation pour la Recherche Médicale. J.F. was an EMBO Fellow.

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