High-throughput dielectrophoretic cell sorting assisted by cell sliding on scalable electrode tracks made of conducting-PDMS
Abstract
Dielectrophoresis (DEP) as a label-free cell separation approach in microdevices has been extensively investigated for a variety of applications. 3D microelectrodes made of conducting-PDMS inherit the merit of volumetric electrodes for generating influential DEP force throughout the entire channel depth and meanwhile, exploit low-cost fabrication process by soft lithography. However, the configuration of conducting-PDMS electrodes is limited to being embedded in sidewall of flow chamber, which leads to rather low flow rate and difficulties in extension of the flow rate. We previously reported a more effective configuration with 3D interdigitated electrodes made of silicon that assist cell sliding along solid tracks, yet such device requires expensive silicon dry etching and, moreover, the track appears to be patterned with non-straight and wavy outline, which not only hinders the flow rate but also allows cell sliding to occur only along its downstream side. Here we demonstrate low-cost silver-PDMS electrode-track featuring ideally straight outline that induces rather uniform drag to drive smooth cell sliding. Such design achieves live and dead cell separation at flow rate twice as that of silicon tracks with cell loading concentration 10 times higher. It also fully utilizes the track to enable cell sliding on both of the up- and down-stream sides. Notably, we also demonstrate that this track is expandable to be V-shape for more advanced bidirectional cell sliding, which is showcased here by tumor cells separation from lymphocytes at 1.2 mL/h. Such results greatly enhance the throughput as compared to the state-of-art conducting-PDMS based cell separator.
Additional Information
© 2020 Elsevier. Received 23 March 2020, Revised 14 August 2020, Accepted 7 September 2020, Available online 12 September 2020. This work was supported by the National Natural Science Foundation of China (Grant No. 61804007), the Fundamental Research Funds for the Central Universities (Grant No. BUCTRC201809 and No. XK1802-4), the Research Funds to the top scientific and technological innovation team from Beijing University of Chemical Technology (Grant No. BUCTYLKJCX06) and the Research Funds from Beijing Advanced Innovation Center for Soft Matter Science and Engineering (Grant No. BAIC201607). CRediT authorship contribution statement: Duli Yu: Funding acquisition, Supervision. Xiaoxing Xing: Funding acquisition Supervision. Declaration of Competing Interest: None.Attached Files
Supplemental Material - 1-s2.0-S092540052031220X-mmc1.pdf
Supplemental Material - 1-s2.0-S092540052031220X-mmc2.mp4
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Additional details
- Eprint ID
- 105374
- DOI
- 10.1016/j.snb.2020.128873
- Resolver ID
- CaltechAUTHORS:20200914-111809586
- 61804007
- National Natural Science Foundation of China
- BUCTRC201809
- Fundamental Research Funds for the Central Universities
- XK1802-4
- Fundamental Research Funds for the Central Universities
- BUCTYLKJCX06
- Beijing University of Chemical Technology
- BAIC201607
- Beijing Advanced Innovation Center for Soft Matter Science and Engineering
- Created
-
2020-09-14Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field