Local auxin biosynthesis is required for root regeneration after wounding
Abstract
The root meristem can regenerate following removal of its stem-cell niche by recruitment of remnant cells from the stump. Regeneration is initiated by rapid accumulation of auxin near the injury site but the source of this auxin is unknown. Here, we show that auxin accumulation arises from the activity of multiple auxin biosynthetic sources that are newly specified near the cut site and that their continuous activity is required for the regeneration process. Auxin synthesis is highly localized while PIN-mediated transport is dispensable for auxin accumulation and tip regeneration. Roots lacking the activity of the regeneration competence factor ERF115, or that are dissected at a zone of low regeneration potential, fail to activate local auxin sources. Remarkably, restoring auxin supply is sufficient to confer regeneration capacity to these recalcitrant tissues. We suggest that regeneration competence relies on the ability to specify new local auxin sources in a precise temporal pattern.
Additional Information
© 2020 Springer Science and Business Media LLC. Received 15 August 2019; Accepted 02 July 2020; Published 03 August 2020. We thank J. Alonso, A. Stepanova and L. de Veylder for sharing research material, Ken-ichiro Hayashi for providing yucasin DF, M. De Martino and A. Lepar for cloning and nightly assistance, and Y. Eshed and S. Savaldi-Goldstein for comments and discussions. I.E. is supported by the Israeli Science Foundation (grant no. ISF966/17) and the Howard Hughes Medical Institute International Research Scholar Grant (grant no. 55008730). Data availability: RNA-seq data are available via GEO (series GSE145488). Author Contributions: R.M. and I.E. conceived and designed the study. R.M., I.C., N.G.Y., A.M. and I.E. carried out the experiments. L.F.S. generated the pGL2:iaaH line. C.V. and E.S. generated pin mutants. R.M. and I.E. wrote the paper. The authors declare no competing interests.Attached Files
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Additional details
- Eprint ID
- 104906
- DOI
- 10.1038/s41477-020-0737-9
- Resolver ID
- CaltechAUTHORS:20200811-093658118
- ISF966/17
- Israel Science Foundation
- 55008730
- Howard Hughes Medical Institute
- Created
-
2020-08-11Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field