Drosophila Aurora A kinase is required to localize D-TACC to centrosomes and to regulate astral microtubules
Abstract
Disruption of the function of the A-type Aurora kinase of Drosophila by mutation or RNAi leads to a reduction in the length of astral microtubules in syncytial embryos, larval neuroblasts, and cultured S2 cells. In neuroblasts, it can also lead to loss of an organized centrosome and its associated aster from one of the spindle poles, whereas the centrosome at the other pole has multiple centrioles. When centrosomes are present at the poles of aurA mutants or aurA RNAi spindles, they retain many antigens but are missing the Drosophila counterpart of mammalian transforming acidic coiled coil (TACC) proteins, D-TACC. We show that a subpopulation of the total Aurora A is present in a complex with D-TACC, which is a substrate for the kinase. We propose that one of the functions of Aurora A kinase is to direct centrosomal organization such that D-TACC complexed to the MSPS/XMAP215 microtubule-associated protein may be recruited, and thus modulate the behavior of astral microtubules.
Additional Information
© 2002 The Rockefeller University Press. After the Initial Publication Period, RUP will grant to the public the non-exclusive right to copy, distribute, or display the Article under a Creative Commons Attribution-Noncommercial-Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode, or updates thereof. Received: 27 August 2001. Revised: 21 December 2001. Accepted: 24 December 2001. We would especially like to thank Adelaide Carpenter for her skilled tuition in carrying out electron microscopy on Drosophila larval brains, and Endre Mathe for helpful advice and stimulating discussions. We would like to thank the Cancer Research Campaign for a Program Grant to D.M. Glover. R. Giet was supported by Fellowships from the Marie Curie Program of the EEEC and the Human Frontiers Science Program. C. Prigent is supported by the Centre National de la Recherche Scientifique, L'association pour la Recherche sur le Cancer, and the Ligue Nationale Contre le Cancer. S. Deschamps is a fellow of the Ligue Nationale Contre le Cancer. J.W. Raff thanks the Wellcome Trust for a Senior Research Fellowship in Basic Biomedical Sciences, and M.J. Lee thanks the Medical Research Council for a research studentship.Attached Files
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Additional details
- PMCID
- PMC2173350
- Eprint ID
- 104854
- Resolver ID
- CaltechAUTHORS:20200807-170352216
- Cancer Research Campaign
- Marie Curie Fellowship
- Human Frontier Science Program
- Centre National de la Recherche Scientifique (CNRS)
- L'association pour la Recherche sur le Cancer
- Ligue Nationale Contre le Cancer
- Wellcome Trust
- Medical Research Council (UK)
- Created
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2020-08-13Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field