Localization of Pavarotti-KLP in Living Drosophila Embryos Suggests Roles in Reorganizing the Cortical Cytoskeleton during the Mitotic Cycle
Abstract
Pav-KLP is the Drosophila member of the MKLP1 family essential for cytokinesis. In the syncytial blastoderm embryo, GFP-Pav-KLP cyclically associates with astral, spindle, and midzone microtubules and also to actomyosin pseudocleavage furrows. As the embryo cellularizes, GFP-Pav-KLP also localizes to the leading edge of the furrows that form cells. In mononucleate cells, nuclear localization of GFP-Pav-KLP is mediated through NLS elements in its C-terminal domain. Mutants in these elements that delocalize Pav-KLP to the cytoplasm in interphase do not affect cell division. In mitotic cells, one population of wild-type GFP-Pav-KLP associates with the spindle and concentrates in the midzone at anaphase B. A second is at the cell cortex on mitotic entry and later concentrates in the region of the cleavage furrow. An ATP binding mutant does not localize to the cortex and spindle midzone but accumulates on spindle pole microtubules to which actin is recruited. This leads either to failure of the cleavage furrow to form or later defects in which daughter cells remain connected by a microtubule bridge. Together, this suggests Pav-KLP transports elements of the actomyosin cytoskeleton to plus ends of astral microtubules in the equatorial region of the cell to permit cleavage ring formation.
Additional Information
© 2003 by The American Society for Cell Biology. Submitted April 8, 2003; Revised May 28, 2003; Accepted May 28, 2003. We thank Matthew Savoian for helpful comments on the manuscript. Cancer Research UK and the European Union provided support for this work.Attached Files
Published - mbc.e03-04-0214.pdf
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Additional details
- PMCID
- PMC206997
- Eprint ID
- 104846
- Resolver ID
- CaltechAUTHORS:20200807-170351387
- Cancer Research UK
- European Union
- Created
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2020-08-11Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field