Analysis of the Drosophila rDNA promoter by transient expression
- Creators
- Hayward, David C.
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Glover, David M.
Abstract
We have examined the expression of the bacterial gene chloramphenicol acetyl transferase (CAT) under the control of the Drosophila rDNA promoter following transfection into Drosophila tissue culture cells. Constructs having an entire NTS, corresponding to approximately 3640 base pairs of upstream rDNA sequence, or constructs with 306 base pairs of upstream sequence respectively, are transcribed at 5 fold or 2 fold higher levels than a construct with 43 base pairs of upstream DNA. In co-transfection experiments, the construct with the entire NTS competes for transcription 20 fold more effectively than the construct with 306 base pairs of upstream sequence. Constructs having either 72 base pairs or 60 base pairs of upstream rDNA sequences, on the other hand, are transcribed very much less efficiently than constructs with either 306 bp or with only 43 bp of upstream DNA. These sequences, which reduce levels of rDNA transcription in the absence of additional upstream DNA, lie in a region in which the rDNA promoter differs from its duplications within the NTS.
Additional Information
© 1988 IRL Press Limited. Creative Commons Attribution Non-Commercial licence (CC BY-NC). Received March 3, 1988; Revised and Accepted April 22, 1988. We would like to thank Peter Rae and Bruce Kohorn for providing us with plasmids. We are grateful to the Cancer Research Campaign for supporting all aspects of this work including a Career Development Award to DMG and a Research Studentship to DCH.Attached Files
Published - nar00153-0073.pdf
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Additional details
- PMCID
- PMC336628
- Eprint ID
- 104844
- Resolver ID
- CaltechAUTHORS:20200807-170351129
- Cancer Research Campaign
- Created
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2020-08-11Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field