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Published May 2012 | Published + Supplemental Material
Journal Article Open

The chromosomal passenger complex controls the function of endosomal sorting complex required for transport-III Snf7 proteins during cytokinesis

Abstract

Cytokinesis controls the proper segregation of nuclear and cytoplasmic materials at the end of cell division. The chromosomal passenger complex (CPC) has been proposed to monitor the final separation of the two daughter cells at the end of cytokinesis in order to prevent cell abscission in the presence of DNA at the cleavage site, but the precise molecular basis for this is unclear. Recent studies indicate that abscission could be mediated by the assembly of filaments comprising components of the endosomal sorting complex required for transport-III (ESCRT-III). Here, we show that the CPC subunit Borealin interacts directly with the Snf7 components of ESCRT-III in both Drosophila and human cells. Moreover, we find that the CPC's catalytic subunit, Aurora B kinase, phosphorylates one of the three human Snf7 paralogues—CHMP4C—in its C-terminal tail, a region known to regulate its ability to form polymers and associate with membranes. Phosphorylation at these sites appears essential for CHMP4C function because their mutation leads to cytokinesis defects. We propose that CPC controls abscission timing through inhibition of ESCRT-III Snf7 polymerization and membrane association using two concurrent mechanisms: interaction of its Borealin component with Snf7 proteins and phosphorylation of CHMP4C by Aurora B.

Additional Information

© 2012 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/3.0/, which permits unrestricted use, provided the original author and source are credited. Received: 21 March 2012. Accepted: 12 April 2012. We thank E. Ferreira, K. Imakhlaf and A. Rousseau for their help during the course of this work, and C. Crump, B. Earnshaw, C. Lindon, S. Ruchaud, A. Sagona, H. Stenmark, and G. Tzolovsky for reagents. We also thank M. Mishima for the midbody purification protocol. This work was supported by two CR-UK project grant (nos. C12296/A8039 and C12296/A12541) and a Newton Trust Research grant to P.P.D. Z.I.B. is supported by a Gwynaeth Pretty PhD fellowship of the Department of Pathology of the University of Cambridge. Work in the Glover laboratory was supported by a CR-UK programme grant.

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August 19, 2023
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