The Pentameric Nucleoplasmin Fold Is Present in Drosophila FKBP39 and a Large Number of Chromatin-Related Proteins
Abstract
Nucleoplasmin is a histone chaperone that consists of a pentameric N-terminal domain and an unstructured C-terminal tail. The pentameric core domain, a doughnut-like structure with a central pore, is only found in the nucleoplasmin family. Here, we report the first structure of a nucleoplasmin-like domain (NPL) from the unrelated Drosophila protein, FKBP39, and we present evidence that this protein associates with chromatin. Furthermore, we show that two other chromatin proteins, Arabidopsis thaliana histone deacetylase type 2 (HD2) and Saccharomyces cerevisiae Fpr4, share the NPL fold and form pentamers, or a dimer of pentamers in the case of HD2. Thus, we propose a new family of proteins that share the pentameric nucleoplasmin-like NPL domain and are found in protists, fungi, plants and animals.
Additional Information
© 2015 The Authors. Published by Elsevier Under a Creative Commons license - Attribution 4.0 International (CC BY 4.0) Received 21 October 2014, Revised 17 March 2015, Accepted 17 March 2015, Available online 24 March 2015. We are grateful to Gunter Stier for providing the vector; Michael Nilges, Oleg Fedorov, Benjamin Bardiaux, Stefanie Hartmann and Wolfgang Rieping for helpful discussions; and Daniel Nietlispach for NMR expertise. We thank Renato Paro for generously providing us with an anti-FKBP39 antibody. We would like to thank the Wellcome Trust for financial support (grant 082010/Z/07/Z). V.T.F. and E.D.L. acknowledge support from Engineering and Physical Sciences Research Council under grants GR/R99393/01 and EP/C015452/1 for the creation of the Deuteration Laboratory platform operating within the Grenoble Partnership for Structural Biology. V.T.F. also acknowledges support from the European Union under contract RII3-CT-2003-505925. J.B.A. acknowledges the provision of a postdoctoral fellowship held at Keele University. M.R.P. and D.M.G. were supported by the Medical Research Council and Cancer Research UK grants to D.M.G. A.A.W. is a recipient of a Wellcome Trust Fellowship 092441/Z/10/Z. J.D. and M.D. were supported by the Harmonia 5 Grant 2013/10/M/NZ2/00298 from the Polish National Science Center. The authors would like to thank the Institut Laue-Langevin (ILL), the European Synchrotron Radiation Facility (ESRF) and the European Molecular Biology Laboratory Hamburg outstation (EMBL-HH) for the provision of beamtime and access to the experimental facilities of D22, ID14eh3 and X33 respectively. We would also like to thank the local contacts at all the facilities for providing assistance in using the beam lines.Attached Files
Published - 1-s2.0-S0022283615001941-main.pdf
Supplemental Material - 1-s2.0-S0022283615001941-mmc1.pdf
Supplemental Material - 1-s2.0-S0022283615001941-mmc2.pdf
Supplemental Material - 1-s2.0-S0022283615001941-mmc3.xls
Supplemental Material - 1-s2.0-S0022283615001941-mmc4.pdf
Supplemental Material - 1-s2.0-S0022283615001941-mmc5.pdf
Supplemental Material - 1-s2.0-S0022283615001941-mmc6.pdf
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Additional details
- PMCID
- PMC4414354
- Eprint ID
- 104790
- Resolver ID
- CaltechAUTHORS:20200806-163115453
- 082010/Z/07/Z
- Wellcome Trust
- GR/R99393/01
- Engineering and Physical Sciences Research Council (EPSRC)
- EP/C015452/1
- Engineering and Physical Sciences Research Council (EPSRC)
- RII3-CT-2003-505925
- European Union
- Keele University
- Medical Research Council (UK)
- Cancer Research UK
- 092441/Z/10/Z
- Wellcome Trust
- 2013/10/M/NZ2/00298
- National Science Centre (Poland)
- Created
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2020-08-10Created from EPrint's datestamp field
- Updated
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2021-11-16Created from EPrint's last_modified field