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Published August 12, 2020 | Accepted Version + Supplemental Material
Journal Article Open

Photoaffinity Probes for the Identification of Sequence-Specific Glycosaminoglycan-Binding Proteins

Abstract

Glycosaminoglycan (GAG)–protein interactions mediate critical physiological and pathological processes, such as neuronal plasticity, development, and viral invasion. However, mapping GAG–protein interaction networks is challenging as these interactions often require specific GAG sulfation patterns and involve transmembrane receptors or extracellular matrix-associated proteins. Here, we report the first GAG polysaccharide-based photoaffinity probes for the system-wide identification of GAG-binding proteins in living cells. A general platform for the modular, efficient assembly of various chondroitin sulfate (CS)-based photoaffinity probes was developed. Systematic evaluations led to benzophenone-containing probes that efficiently and selectively captured known CS-E-binding proteins in vitro and in cells. Importantly, the probes also enabled the identification of >50 new proteins from living neurons that interact with the neuroplasticity-relevant CS-E sulfation motif. Several candidates were independently validated and included membrane receptors important for axon guidance, innate immunity, synapse development, and synaptic plasticity. Overall, our studies provide a powerful approach for mapping GAG–protein interaction networks, revealing new potential functions for these polysaccharides and linking them to diseases such as Alzheimer's and autism.

Additional Information

© 2020 American Chemical Society. Received: June 3, 2020; Published: July 27, 2020. We thank Dr. Michael Sweredoski, Dr. Annie Moradian, and Dr. Brett Lomenick from the Protein Exploration Laboratory (PEL) at Caltech. This work was supported by the NIH (R01 GM093627). The authors declare no competing financial interest.

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Accepted Version - nihms-1637051.pdf

Supplemental Material - ja0c06046_si_001.pdf

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August 19, 2023
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